Customer-supplied antibody or protein
Submit the sample type, amount, concentration, buffer and application. Process and delivery boundaries are confirmed after feasibility assessment.
Submit sample informationFLUORESCENT LABELING & CONJUGATION
Fluorescent Labeling & Conjugation
Fluorescent-labeling projects for antibodies, fragments and recombinant proteins, from sample assessment and dye selection through conjugation, purification and agreed basic quality checks.
SERVICE BOUNDARYThis page describes the project workflow and assessable capabilities. It does not prescribe sample amount, timing or fixed quality tests; the formal scope is defined by technical assessment and project confirmation.
ENGAGEMENT MODE
Two ways to start a project
Label customer-supplied material, or begin by assessing confirmed StellaCyte material or a development candidate.
StellaCyte material or development candidate
Request a combination using available dyes, confirmed material or an antibody-development candidate. Expression, supply and labeling status are assessed separately.
View products and candidatesSERVICE SCOPE
Labeling projects available for assessment
Build practical service combinations around StellaCyte antibodies, recombinant proteins, fluorescent dyes and conjugation processes.
Fluorescent antibody labeling
Single-color labeling of research-grade IgG and biosimilar antibodies for flow cytometry, imaging and binding analysis.
UV421 · SF488 · SF594 · PE · APC · other options by assessmentAntibody fragments and recombinant antibodies
Assess the labeling route from molecular structure, available reactive sites and downstream application, with recovery and risks confirmed before project initiation.
Fab / F(ab')₂ · Recombinant antibodyFluorescent labeling of recombinant proteins
Protein labeling for tracking, binding, internalization and cell analysis, with sample amount and buffer assessed against protein properties.
Sample composition and buffer · project assessmentBiotin and other small-molecule conjugation
Discuss biotinylation or other custom conjugation after functional-group and process-feasibility review.
NHS · Maleimide · Other chemistryPROJECT WORKFLOW
Five steps from sample to delivery
Each step includes a confirmation point so inputs, risks and delivery boundaries are clear before experimental work begins.
- 01
Request and sample assessment
Application, sample type, amount, purity and buffer system
- 02
Dye and process selection
Recommendation based on instrument channels, target abundance and reactive sites
- 03
Conjugation reaction
Small-scale or project-scale labeling under the confirmed plan
- 04
Purification and basic quality checks
Remove free dye and complete the agreed basic assessments
- 05
Delivery and technical record
Deliver material, a results summary and agreed project records
SAMPLE GUIDE
Sample information needed for assessment
- Sample amount and concentration
- Provide the available amount and concentration for assessment against the molecule and process
- Purity and analytical method
- Provide available purity results and the analytical method used
- Buffer system
- Disclose amines, sodium azide, glycerol, salts, reducing agents and other components
- Sample details
- Molecular type, molecular weight, application and storage conditions
- Requested timing
- Provide the requested window; the project schedule is confirmed after assessment
PROJECT LEVELS
Choose the engagement depth by project maturity
This is a discussion framework, not a fixed package. Actual work depends on sample properties and intended use.
Feasibility assessment
For projects with incomplete sample conditions that need an initial reaction-route and risk assessment.
- Sample and buffer review
- Reactive-site and dye recommendation
- Risk and missing-information list
Standard labeling project
For research projects with a clear target label and application that can use an established process route.
- Plan confirmation and conjugation
- Free-dye removal
- Agreed basic-results summary
Custom process project
For special molecular formats, labels, recovery targets or analytical requirements that need dedicated development.
- Process-condition exploration
- Purification and analytical-method alignment
- Stage results and delivery confirmation
BUFFER REVIEW
View the sample and buffer guideBuffer pre-check
Conjugation chemistries have different constraints. The following supports sample-submission discussion and is not an unevaluated operating protocol.
Component or informationAssessment noteRecommended action
Protein concentration and total volumeDetermines reaction scale, recovery assessment and analytical methodRequired
Amine-containing components such as Tris or glycineMay interfere with some NHS reaction routesDisclose and assess buffer exchange
Carrier proteins such as BSA or gelatinMay react with the target molecule or affect quantificationRemoval usually recommended
Sodium azide, glycerol and salt concentrationMay affect process, purification or downstream useDisclose fully
Reducing agents such as DTT or TCEPMay affect thiol or protein-structure-related routesRequired disclosure
Molecular weight, purity and storage conditionsUsed to select purification and assess sample stabilityRequired
START A PROJECT
Submit a labeling requestTell us about the sample, requested label and application
You can submit the information currently available. The technical team will identify additional sample and process details needed for assessment.